Issue 7, 2023

Visible-light driven fumarate synthesis from pyruvate and gaseous CO2 with a hybrid system of photocatalytic NADH regeneration and dual biocatalysts

Abstract

Fumarate is a useful unsaturated dicarboxylate utilized as a precursor for unsaturated polyester resin and biodegradable plastics. Fumarate is partially produced from petroleum-derived materials; thus, it is necessary to establish a synthesis from renewable raw materials such as gaseous CO2 and biobased compounds with an external renewable energy source such as solar light. In this work, the visible-light driven synthesis of fumarate from biobased pyruvate is reported, which uses CO2 directly captured from the gas phase by a 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)–NaOH buffer solution in combination with the NAD+ reduction system of triethanolamine (TEOA), water-soluble zinc porphyrin, zinc meso-tetra(4-sulfonatophenyl) porphyrin tetrasodium salt (ZnTPPS) and the Rh coordination complex ([Cp*Rh(bpy)(H2O)]2+; Cp* = pentamethylcyclopentadienyl, bpy = 2,2′-bipyridyl). In addition, dual-biocatalysts consisting of malate dehydrogenase (oxaloacetate-decarboxylating; MDH; EC 1.1.1.38) from Sulfobus tokodaii and fumarase from porcine heart (FUM; EC 4.2.1.2) were used. It was found that pyruvate can be converted into L-malate with MDH by directly using CO2 gas as a carboxylating agent in the presence of NADH. Moreover, the development of visible-light driven fumarate synthesis from gaseous CO2 and pyruvate, employing a system with NADH regeneration and dual-biocatalysts as raw materials was also established.

Graphical abstract: Visible-light driven fumarate synthesis from pyruvate and gaseous CO2 with a hybrid system of photocatalytic NADH regeneration and dual biocatalysts

Supplementary files

Article information

Article type
Paper
Submitted
15 Jun 2023
Accepted
06 Sep 2023
First published
07 Sep 2023
This article is Open Access
Creative Commons BY-NC license

RSC Sustain., 2023,1, 1874-1882

Visible-light driven fumarate synthesis from pyruvate and gaseous CO2 with a hybrid system of photocatalytic NADH regeneration and dual biocatalysts

M. Takeuchi and Y. Amao, RSC Sustain., 2023, 1, 1874 DOI: 10.1039/D3SU00194F

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