Issue 27, 2023

Synthesis of oligosaccharides to identify an immunologically active epitope against Candida auris infection

Abstract

Candida auris (C. auris) is an emerging multidrug-resistant fungal pathogen that represents a significant public health challenge as it can spread rapidly and result in high mortality rates. The mannans on the C. auris cell surface are potent immunogens and attractive targets for developing a glycoconjugate vaccine. We synthesized the oligosaccharides resembling cell surface mannans of C. auris and printed them onto microarray slides that were used to screen plasma from mice infected with C. auris. IgM antibodies in mouse plasma recognize the β-1,2 linkage present in C. auris surface mannans. Disaccharide 19 emerged from glycan array screening as a lead for developing a vaccine against C. auris, as the majority of patient plasma samples showed antibodies against this glycan. The synthetic oligosaccharides can be used for the early detection of C. auris infections.

Graphical abstract: Synthesis of oligosaccharides to identify an immunologically active epitope against Candida auris infection

Supplementary files

Article information

Article type
Edge Article
Submitted
07 Mar 2023
Accepted
16 Jun 2023
First published
23 Jun 2023
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2023,14, 7559-7563

Synthesis of oligosaccharides to identify an immunologically active epitope against Candida auris infection

R. K. Singh, E. E. Reuber, M. Bruno, M. G. Netea and P. H. Seeberger, Chem. Sci., 2023, 14, 7559 DOI: 10.1039/D3SC01242E

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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