Issue 16, 2023

Simultaneous observation of the spatial and temporal dynamics of single enzymatic catalysis using a solid-state nanopore

Abstract

We developed a bipolar SiNx nanopore for the observation of single-molecule heterogeneous enzymatic dynamics. Single glucose oxidase was immobilized inside the nanopore and its electrocatalytic behaviour was real-time monitored via continuous recording of ionic flux amplification. The temporal heterogeneity in enzymatic properties and its spatial dynamic orientations were observed simultaneously, and these two properties were found to be closely correlated. We anticipate that this method offers new perspectives on the correlation of protein structure and function at the single-molecule level.

Graphical abstract: Simultaneous observation of the spatial and temporal dynamics of single enzymatic catalysis using a solid-state nanopore

Supplementary files

Article information

Article type
Communication
Submitted
14 Nov 2022
Accepted
14 Mar 2023
First published
15 Mar 2023

Nanoscale, 2023,15, 7261-7266

Simultaneous observation of the spatial and temporal dynamics of single enzymatic catalysis using a solid-state nanopore

R. Yu, Q. Li, S. Liu, H. Ma, Y. Ying and Y. Long, Nanoscale, 2023, 15, 7261 DOI: 10.1039/D2NR06361A

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