Peroxidase mimicking activity of Saccharum officinarum L. capped gold nanoparticles using o-dianisidine as a substrate

Abstract

In this study, a biogenic method is reported for the fabrication of gold nanoparticles (Au NPs) using Saccharum officinarum L. (SOL) as a dual performer, both as a reducing and stabilizing agent. The method used in this study is efficient, economic, and eco-friendly for the fabrication of noble metals NPs. The peroxidase mimicking activity of the SOL capped Au NPs (S-Au NPs) was evaluated using o-dianisidine (ODA) as a substrate and Au NPs as an enzyme mimic. A color change from colorless to brown was observed for the oxidized ODA product with a UV-visible peak at 420 nm in the presence of Au NPs and H₂O₂. An enhanced enzyme activity of 95.7% was observed for the Au NPs. Kinetic activity was evaluated by Michaelis–Menten plot, and the k_m and V_max values were found to be 0.62 mM and 0.20 min⁻¹, respectively. The detailed probable mechanism of Au NPs synthesis and the oxidation of ODA with S-Au NPs were also discussed. This novel synthetic method provides a sustainable route for the synthesis of diverse NPs in future and various biogenic NPs can be explored as an efficient nanoenzyme in the drug delivery and sensing field.