ARCHE-NOAH: NMR supersequence with five different CEST experiments for studying protein conformational dynamics

Abstract

An NMR NOAH-supersequence is presented consisting of five CEST experiments for studying protein backbone and side-chain dynamics by ¹⁵N-CEST, carbonyl-¹³CO-CEST, aromatic-¹³C_ar-CEST, ¹³C_α-CEST, and methyl-¹³C_met-CEST. The new sequence acquires the data for these experiments in a fraction of the time required for the individual experiments, saving over four days of NMR time per sample.