Issue 27, 2023

DNA labelling in live cells via visible light-induced [2+2] photocycloaddition

Abstract

We introduce a visible light-driven (λmax = 451 nm) photo-chemical strategy for labelling of DNA in living HeLa cells via the [2+2] cycloaddition of a styrylquinoxaline moiety, which we incorporate into both the DNA and the fluorescent label. Our methodology offers advanced opportunities for the mild remote labelling of DNA in water while avoiding UV light activation.

Graphical abstract: DNA labelling in live cells via visible light-induced [2+2] photocycloaddition

Supplementary files

Article information

Article type
Communication
Submitted
21 Feb 2023
Accepted
02 Mar 2023
First published
02 Mar 2023

Chem. Commun., 2023,59, 4012-4015

DNA labelling in live cells via visible light-induced [2+2] photocycloaddition

R. T. Michenfelder, L. Delafresnaye, V. X. Truong, C. Barner-Kowollik and H. Wagenknecht, Chem. Commun., 2023, 59, 4012 DOI: 10.1039/D3CC00817G

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