Fluorescence-based chemical tools for monitoring ultrasound-induced hydroxyl radical production in aqueous solution and in cells

Cherie CY. Wong; Lu-Lu Sun; Meng-Jiao Liu; Eleanor Stride; Jason L. Raymond; Hai-Hao Han; James Kwan; Adam C. Sedgwick

doi:10.1039/D3CC00364G

Fluorescence-based chemical tools for monitoring ultrasound-induced hydroxyl radical production in aqueous solution and in cells†

Cherie CY. Wong,^a Lu-Lu Sun,^b Meng-Jiao Liu,^c Eleanor Stride,

^d Jason L. Raymond,

^a Hai-Hao Han,

*^bc James Kwan

*^a and Adam C. Sedgwick

*^e

Author affiliations

* Corresponding authors

^a Department of Engineering Science, Parks Road, Oxford, UK
E-mail: james.kwan@balliol.ox.ac.uk

^b Shandong Laboratory of Yantai Drug Discovery, Bohai Rim Advanced Research Institute for Drug Discovery, Yantai, Shandong, P. R. China
E-mail: hanhaihao@simm.ac.cn

^c Molecular Imaging Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, P. R. China

^d Institute of Biomedical Engineering, Department of Engineering Sciences, Old Road Campus Research Building, University of Oxford, Headington, Oxford, UK

^e Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Mansfield Road, UK
E-mail: adam.sedgwick@chem.ox.ac.uk

Abstract

We report the synthesis of hydroxyl-radical (˙OH) responsive fluorescent probes that utilise the 3,5-dihydroxybenzyl (DHB) functionality. 4-Methylumbeliferone-DHB (Umb-DHB) and resorufin-DHB (Res-DHB) in the presence of ˙OH radicals resulted in significant increases in their respective fluorescent emission intensities at 460 nm and 585 nm. The incubation of Res-DHB in HeLa cells followed by therapeutic ultrasound (1 MHz) resulted in a significant increase in fluorescence emission intensity thus permitting the ability to monitor ultrasound-induced ˙OH production in live cells.

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DOI: https://doi.org/10.1039/D3CC00364G
Article type: Communication
Submitted: 26 Jan 2023
Accepted: 15 Mar 2023
First published: 21 Mar 2023
This article is Open Access

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Chem. Commun., 2023,59, 4328-4331

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Fluorescence-based chemical tools for monitoring ultrasound-induced hydroxyl radical production in aqueous solution and in cells

C. CY. Wong, L. Sun, M. Liu, E. Stride, J. L. Raymond, H. Han, J. Kwan and A. C. Sedgwick, Chem. Commun., 2023, 59, 4328 DOI: 10.1039/D3CC00364G

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Chemical Communications

Fluorescence-based chemical tools for monitoring ultrasound-induced hydroxyl radical production in aqueous solution and in cells†

Abstract

Supplementary files

Article information

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Fluorescence-based chemical tools for monitoring ultrasound-induced hydroxyl radical production in aqueous solution and in cells

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