Issue 27, 2023
From the journal:

Chemical Communications

Total chemical synthesis of sumoylated histone H4 reveals negative biochemical crosstalk with histone ubiquitylation†

Sumeet K. Singh, ORCID logo a   Andres Reyna,a   Xiaowen Xie, ORCID logo b   Haibin Mao,b   Meihuan Ji, ORCID logo a   Ning Zheng,b   Peter L. Hsu‡b  and  Champak Chatterjee ORCID logo *a  

* Corresponding authors

a Department of Chemistry, University of Washington, Seattle 98195, USA
E-mail: champak1@uw.edu

b Department of Pharmacology, University of Washington; Howard Hughes Medical Institute, University of Washington, Seattle 98195, USA

Abstract

An efficient total chemical synthesis of site-specifically sumoylated histone H4 was undertaken to generate homogenously modified mononucleosomes. These were tested as substrates in biochemical assays with the histone H2B-specific ubiquitin ligases Rad6 and Bre1, which revealed the strong inhibition of H2B ubiquitylation by SUMO. This novel negative biochemical crosstalk between SUMO and ubiquitin was also confirmed to exist in human cells.

Graphical abstract: Total chemical synthesis of sumoylated histone H4 reveals negative biochemical crosstalk with histone ubiquitylation

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Article information

DOI
https://doi.org/10.1039/D2CC06683A
Article type
Communication
Submitted
09 Dec 2022
Accepted
07 Mar 2023
First published
09 Mar 2023

Chem. Commun., 2023,59, 4063-4066

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Total chemical synthesis of sumoylated histone H4 reveals negative biochemical crosstalk with histone ubiquitylation

S. K. Singh, A. Reyna, X. Xie, H. Mao, M. Ji, N. Zheng, P. L. Hsu and C. Chatterjee, Chem. Commun., 2023, 59, 4063 DOI: 10.1039/D2CC06683A

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