Long-term imaging of intranuclear Mg2+ dynamics during mitosis using a localized fluorescent probe

Yusuke Matsui; Toshiyuki Kowada; Yi Ding; Priya Ranjan Sahoo; Kazuya Kikuchi; Shin Mizukami

doi:10.1039/D2CC05930D

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Long-term imaging of intranuclear Mg²⁺ dynamics during mitosis using a localized fluorescent probe†

Yusuke Matsui,‡^a Toshiyuki Kowada,

‡^bc Yi Ding,^c Priya Ranjan Sahoo,^b Kazuya Kikuchi

*^ad and Shin Mizukami

*^bc

Author affiliations

* Corresponding authors

^a Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan
E-mail: kkikuchi@mls.eng.osaka-u.ac.jp

^b Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai, Miyagi 980-8577, Japan
E-mail: shin.mizukami@tohoku.ac.jp

^c Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980-8577, Japan

^d WPI-Immunology Frontier Research Center, Osaka University, Suita, Osaka 565-0871, Japan

Abstract

A novel fluorescent Mg²⁺ probe was developed based on a small molecule–protein hybrid. This probe enables subcellular targeting, long-term imaging, and high selectivity for Mg²⁺ over Ca²⁺. Using ratiometric fluorescence microscopy with a co-localized standard fluorophore, the variations in intranuclear Mg²⁺ concentrations during mitosis could be visualized.

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Article information

DOI: https://doi.org/10.1039/D2CC05930D
Article type: Communication
Submitted: 02 Nov 2022
Accepted: 10 May 2023
First published: 16 May 2023
This article is Open Access

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Chem. Commun., 2023,59, 7048-7051

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Long-term imaging of intranuclear Mg²⁺ dynamics during mitosis using a localized fluorescent probe

Y. Matsui, T. Kowada, Y. Ding, P. R. Sahoo, K. Kikuchi and S. Mizukami, Chem. Commun., 2023, 59, 7048 DOI: 10.1039/D2CC05930D

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