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A novel fluorescent Mg2+ probe was developed based on a small molecule–protein hybrid. This probe enables subcellular targeting, long-term imaging, and high selectivity for Mg2+ over Ca2+. Using ratiometric fluorescence microscopy with a co-localized standard fluorophore, the variations in intranuclear Mg2+ concentrations during mitosis could be visualized.

Graphical abstract: Long-term imaging of intranuclear Mg2+ dynamics during mitosis using a localized fluorescent probe

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