A tetranuclear polypyridylruthenium(II) complex as a selective stain for extracellular vesicle penetration through brain microvascular endothelium

Kartika Wardhani; Aviva Levina; Biyun Sun; Georges E. R. Grau; F. Richard Keene; J. Grant Collins; Peter A. Lay

doi:10.1039/D2CC05827H

A tetranuclear polypyridylruthenium(ii) complex as a selective stain for extracellular vesicle penetration through brain microvascular endothelium†

Kartika Wardhani,^a Aviva Levina,

^a Biyun Sun,^b Georges E. R. Grau,^cdef F. Richard Keene,

^gh J. Grant Collinsⁱ and Peter A. Lay

*^acdej

Author affiliations

* Corresponding authors

^a School of Chemistry, The University of Sydney, Sydney, NSW, Australia
E-mail: peter.lay@sydney.edu.au

^b Dementia Research Centre, Department of Biomedical Science, Macquarie University, Sydney, NSW, Australia

^c Sydney Nano, The University of Sydney, Sydney, NSW, Australia

^d Sydney Cancer Network, The University of Sydney, Sydney, NSW, Australia

^e Marie Bashir Institute, The University of Sydney, Sydney, NSW, Australia

^f Vascular Immunology Unit, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW, Australia

^g Department of Chemistry, School of Physical Sciences, The University of Adelaide, Adelaide, SA, Australia

^h Australian Institute of Tropical Health and Medicine/Centre for Molecular Therapeutics, James Cook University, Townsville, QLD, Australia

ⁱ School of Science, The University of New South Wales, Australian Defence Force Academy, Canberra, ACT, Australia

^j Sydney Analytical, The University of Sydney, Sydney, NSW, Australia

Abstract

A new photoluminescent polypyridylruthenium(II) stain for extracellular vesicles (EVs) released from lipopolysaccharide-stimulated THP-1 monocytes enabled important new insights into how the bacteria-induced immune system affects the blood–brain barrier (BBB). These included previously unknown aspects of EV interactions with BBB microvascular endothelial cells and the extracellular matrix relevant to human brain diseases.

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Article information

DOI: https://doi.org/10.1039/D2CC05827H
Article type: Communication
Submitted: 27 Oct 2022
Accepted: 05 May 2023
First published: 10 May 2023

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Chem. Commun., 2023,59, 6877-6880

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A tetranuclear polypyridylruthenium(II) complex as a selective stain for extracellular vesicle penetration through brain microvascular endothelium

K. Wardhani, A. Levina, B. Sun, G. E. R. Grau, F. R. Keene, J. G. Collins and P. A. Lay, Chem. Commun., 2023, 59, 6877 DOI: 10.1039/D2CC05827H

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Chemical Communications

A tetranuclear polypyridylruthenium(ii) complex as a selective stain for extracellular vesicle penetration through brain microvascular endothelium†

Abstract

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A tetranuclear polypyridylruthenium(II) complex as a selective stain for extracellular vesicle penetration through brain microvascular endothelium

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