Solid-state NMR – a complementary technique for protein framework characterization

Linda Cerofolini; Kiefer O. Ramberg; Luis C. Padilla; Paweł Antonik; Enrico Ravera; Claudio Luchinat; Marco Fragai; Peter B. Crowley

doi:10.1039/D2CC05725E

Solid-state NMR – a complementary technique for protein framework characterization†

Linda Cerofolini,

^ab Kiefer O. Ramberg,

^c Luis C. Padilla,^ad Paweł Antonik,^c Enrico Ravera,

^abd Claudio Luchinat,

^abd Marco Fragai*^abd and Peter B. Crowley

*^c

Author affiliations

* Corresponding authors

^a Magnetic Resonance Center (CERM), University of Florence, Via L. Sacconi 6, Sesto Fiorentino 50019, Italy
E-mail: fragai@cerm.unifi.it

^b Consorzio Interuniversitario Risonanze Magnetiche di Metalloproteine (CIRMMP), Via L. Sacconi 6, Sesto Fiorentino 50019, Italy

^c School of Biological and Chemical Sciences, University of Galway, University Road, Galway H91 TK33, Ireland
E-mail: peter.crowley@nuigalway.ie

^d Department of Chemistry “Ugo Schiff”, University of Florence, Via della Lastruccia 3, Sesto Fiorentino 50019, Italy

Abstract

Protein frameworks are an emerging class of biomaterial with medical and technological applications. Frameworks are studied mainly by X-ray diffraction or scattering techniques. Complementary strategies are required. Here, we report solid-state NMR analyses of a microcrystalline protein–macrocycle framework and the rehydrated freeze-dried protein. This methodology may aid the characterization of low-crystallinity frameworks.

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DOI: https://doi.org/10.1039/D2CC05725E
Article type: Communication
Submitted: 21 Oct 2022
Accepted: 15 Dec 2022
First published: 15 Dec 2022
This article is Open Access

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Chem. Commun., 2023,59, 776-779

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Solid-state NMR – a complementary technique for protein framework characterization

L. Cerofolini, K. O. Ramberg, L. C. Padilla, P. Antonik, E. Ravera, C. Luchinat, M. Fragai and P. B. Crowley, Chem. Commun., 2023, 59, 776 DOI: 10.1039/D2CC05725E

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Chemical Communications

Solid-state NMR – a complementary technique for protein framework characterization†

Abstract

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Solid-state NMR – a complementary technique for protein framework characterization

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