Monitoring of organophosphorus pesticide residues in plant and vegetable tissues by a novel silver nanocluster probe

Abstract

More and more attention has been paid to the problem of pesticide residues, especially in plants and vegetables, due to their close relationship with human health and food safety. Compared to conventional detecting techniques, the fluorescence sensing method has achieved good results for pesticides detection. However, most of the reported fluorescent probes needed two or more steps to achieve the detection of pesticide residues, which greatly limited their application for in vivo imaging and in situ analysis of agricultural residues in plants and vegetables. In this paper, an Ag nanoclusters (AgNCs) based fluorescent probe is developed for one-step detection of pesticide residues. The novel nanoprobe displayed impressive advantages, such as a selective response to glyphosate pesticide, rapid response within 1 min and an ultralow detection limit of 21 nM. The sensing mechanism is attributed to the unique coordination interaction between AgNCs and glyphosate, which not only increased the size of AgNCs to form big Ag particles, but also caused the fluorescence quenching of AgNCs system. Due to its favorable properties, the probe has been successfully applied to imaging the organophosphorus pesticide of glyphosate in the leave tissues of Arabidopsis thaliana and the root tip cells of lettuce for the first time.