Development and validation of a mass spectrometry based analytical method to quantify the ratios in hemagglutinin trimers in quadrivalent influenza nanoparticle vaccine – FluMos-v1

Asif Shajahan; Cindy X. Cai; Jeremy Wolff; R. Sylvie Yang; Vera B. Ivleva; Daniel B. Gowetski; Jason G. D. Gall; Q. Paula Lei

doi:10.1039/D2AY01890J

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Development and validation of a mass spectrometry based analytical method to quantify the ratios in hemagglutinin trimers in quadrivalent influenza nanoparticle vaccine – FluMos-v1†

Asif Shajahan,‡^a Cindy X. Cai,‡^a Jeremy Wolff,^a R. Sylvie Yang,^a Vera B. Ivleva,^a Daniel B. Gowetski,

^a Jason G. D. Gall

^a and Q. Paula Lei

*^a

Author affiliations

* Corresponding authors

^a Vaccine Production Program Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Gaithersburg, MD, USA
E-mail: paula.lei@nih.gov

Abstract

A quadrivalent influenza nanoparticle vaccine (FluMos-v1) offers long-lasting protection against multiple influenza virus strains and is composed of four strains of hemagglutinin trimer (HAT) assembled around a pentamer core. Here we report an LC-MS/MS analytical development and validation method to measure the percentage of each HAT component in FluMos-v1.

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DOI: https://doi.org/10.1039/D2AY01890J
Article type: Communication
Submitted: 18 Nov 2022
Accepted: 11 Jan 2023
First published: 12 Jan 2023

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Anal. Methods, 2023,15, 896-900

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Development and validation of a mass spectrometry based analytical method to quantify the ratios in hemagglutinin trimers in quadrivalent influenza nanoparticle vaccine – FluMos-v1

A. Shajahan, C. X. Cai, J. Wolff, R. S. Yang, V. B. Ivleva, D. B. Gowetski, J. G. D. Gall and Q. P. Lei, Anal. Methods, 2023, 15, 896 DOI: 10.1039/D2AY01890J

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Analytical Methods

Development and validation of a mass spectrometry based analytical method to quantify the ratios in hemagglutinin trimers in quadrivalent influenza nanoparticle vaccine – FluMos-v1†

Abstract

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Development and validation of a mass spectrometry based analytical method to quantify the ratios in hemagglutinin trimers in quadrivalent influenza nanoparticle vaccine – FluMos-v1

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