A perylenemonoimide-based fluorescent probe: ultrasensitive and selective tracing of endogenous peroxynitrite in living cells

Abstract

Peroxynitrite (ONOO⁻), a highly reactive species, plays a key role in various physiological and pathological processes. Herein, a red-emitting fluorescent reporter perylenemonoimide–boronate ester (PMI-BE) was synthesized and utilized for ultrasensitive detection of ONOO⁻. The unique feature of PMI-BE is its nanomolar sensitivity with high selectivity towards ONOO⁻. Moreover, PMI-BE also detects endogenously generated ONOO⁻ in live cells.