A 3D printable synthetic hydrogel as an immobilization matrix for continuous synthesis with fungal peroxygenases

Abstract

Enzyme immobilization is the key to an intensified bioprocess that allows recycling of the heterogenized enzyme and/or continuous biocatalytic production. In this communication, we present a case study for enzyme immobilization in a novel, 3D printable synthetic hydrogel and its use in continuous oxidation reactions. Immobilization resulted in an average immobilization yield of 6.1% and continuous synthesis was run for 24 hours with a space–time yield of 3.1 × 10⁻² g L⁻¹ h⁻¹.