Engineering exposed vertical nano-TiO2 (001) facets/BiOI nanosheet heterojunction film for constructing a satisfactory PEC glucose oxidase biosensor

Abstract

In the field of photoelectrochemical (PEC) enzyme biosensors, constructing efficient photoelectrodes, in which the recombination of photogenerated carriers is an important factor affecting the performance, is of great significance. Herein, to enhance the separation efficiency of photogenerated carriers, titanium dioxide (TiO₂) nanosheet (NS)/bismuth oxyiodide (BiOI) NS/glucose oxidase (GOx) composites were prepared via hydrothermal and solvothermal methods. Single-crystal anatase TiO₂ NSs with a high percentage of (001) facets lead to better photocarrier separation due to heterojunctions between facets. After coupling with BiOI NSs, the photoelectrochemical performance of the electrode was greatly improved. The photogenerated electrons from TiO₂ and BiOI gathered at TiO₂ (101) and were exported through the fluorine-doped tin oxide (FTO) substrate to generate electrical signals. Photogenerated holes were transferred to TiO₂ (001) and BiOI to participate in the enzymatic reaction, showing the outstanding separation of electrons and holes. The prepared TiO₂ NS/BiOI NS/GOx glucose biosensor achieved satisfactory results, with sensitivity of 14.25 μA mM⁻¹ cm⁻², a linear measurement range of 0–1 mM, and a limit of detection (3S/N) of 0.01 mM in phosphate buffered saline (PBS) at a pH of 7.4. The mechanism for the efficient separation of photogenerated carriers based on the facet heterojunctions introduced in this paper also provides new insights into other optoelectronic biosensors.