Issue 19, 2022

Cryostorage of unstable N-acetylglucosaminyltransferase-V by synthetic zwitterions

Abstract

We report biocompatible materials for cryostorage of unstable proteins such as cancer-related enzyme, N-acetylglucosaminyltransferase-V (GnT-V). GnT-V activity and the amount of protein after freezing were better retained in synthetic zwitterion solutions than in the glycerol solution. This study highlights the potential utility of synthetic zwitterions as novel cryoprotectants.

Graphical abstract: Cryostorage of unstable N-acetylglucosaminyltransferase-V by synthetic zwitterions

Supplementary files

Article information

Article type
Paper
Submitted
10 Mar 2022
Accepted
07 Apr 2022
First published
14 Apr 2022
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2022,12, 11628-11631

Cryostorage of unstable N-acetylglucosaminyltransferase-V by synthetic zwitterions

T. Hirata, T. Takekiyo, Y. Yoshimura, Y. Tokoro, T. Ishizaki, Y. Kizuka and K. Kuroda, RSC Adv., 2022, 12, 11628 DOI: 10.1039/D2RA01575G

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements