Issue 8, 2022, Issue in Progress

Developing a far-red fluorogenic beta-galactosidase probe for senescent cell imaging and photoablation

Abstract

A methylene blue (MB)-based beta-galactosidase (β-gal) activatable molecule, Gal-MB, was developed for senescence imaging and light-triggered senolysis. When in contact with LacZ β-gal or senescence-associated β-gal (SA-β-gal), the photoinsensitive Gal-MB becomes fluorescent. Gal-MB also offered selective phototoxicity toward LacZ β-gal expressing cells and drug-induced senescent cells, which express SA-β-gal, after light illumination at 665 nm.

Graphical abstract: Developing a far-red fluorogenic beta-galactosidase probe for senescent cell imaging and photoablation

Supplementary files

Article information

Article type
Paper
Submitted
18 Jan 2022
Accepted
31 Jan 2022
First published
04 Feb 2022
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2022,12, 4543-4549

Developing a far-red fluorogenic beta-galactosidase probe for senescent cell imaging and photoablation

S. K. Lee, Z. Shen, M. S. Han and C. Tung, RSC Adv., 2022, 12, 4543 DOI: 10.1039/D2RA00377E

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