The substrate scope of dehydratases in antibiotic biosynthesis and their application in kinetic resolutions

Zhiyong Yin; Daniel Bär; Bertolt Gust; Jeroen S. Dickschat

doi:10.1039/D2OB01879A

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The substrate scope of dehydratases in antibiotic biosynthesis and their application in kinetic resolutions†

Zhiyong Yin,^a Daniel Bär,^b Bertolt Gust

^b and Jeroen S. Dickschat

*^a

Author affiliations

* Corresponding authors

^a Kekulé-Institute for Organic Chemistry and Biochemistry, University of Bonn, Gerhard-Domagk-Straße 1, 53121 Bonn, Germany
E-mail: dickschat@uni-bonn.de

^b Pharmaceutical Biology, University of Tübingen, Auf der Morgenstelle 8, 72076 Tübingen, Germany

Abstract

Nine dehydratases involved in the biosynthesis of secondary metabolites in addition to FabZ from fatty acid biosynthesis were investigated for their substrate scope using a panel of N-acetylcysteamine (SNAC) thioesters. The best performing enzyme BorDH2 was applied in kinetic resolutions.

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Article information

DOI: https://doi.org/10.1039/D2OB01879A
Article type: Communication
Submitted: 13 Oct 2022
Accepted: 03 Nov 2022
First published: 04 Nov 2022

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Org. Biomol. Chem., 2022,20, 9103-9107

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The substrate scope of dehydratases in antibiotic biosynthesis and their application in kinetic resolutions

Z. Yin, D. Bär, B. Gust and J. S. Dickschat, Org. Biomol. Chem., 2022, 20, 9103 DOI: 10.1039/D2OB01879A

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Organic & Biomolecular Chemistry

The substrate scope of dehydratases in antibiotic biosynthesis and their application in kinetic resolutions†

Abstract

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The substrate scope of dehydratases in antibiotic biosynthesis and their application in kinetic resolutions

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