Development of an ATP-independent bioluminescent probe for detection of extracellular hydrogen peroxide

Abstract

This work reports a new ATP-independent bioluminescent probe (bor-DTZ) for detecting hydrogen peroxide that is compatible with the Nanoluciferase enzyme. The probe is designed with an arylboronate ester protecting group appended to a diphenylterazine core via a self-immolative phenolate linker. Reaction with hydrogen peroxide reveals diphenylterazine, which can then react with Nanoluciferase to produce a detectable bioluminescent signal. Bor-DTZ shows a dose-dependent response to hydrogen peroxide and selectivity over other biologically relevant reactive oxygen species and can be applied to detect either intra- or extracellular species. We further demonstrate the ability of this platform to monitor fluxes in extracellular hydrogen peroxide in a breast cancer cell line in response to the anticancer treatment, cisplatin.

Graphical abstract: Development of an ATP-independent bioluminescent probe for detection of extracellular hydrogen peroxide

  • This article is part of the themed collection: New Talent

Supplementary files

Article information

Article type
Paper
Submitted
03 Mar 2022
Accepted
05 May 2022
First published
12 May 2022

Org. Biomol. Chem., 2022, Advance Article

Development of an ATP-independent bioluminescent probe for detection of extracellular hydrogen peroxide

J. J. O'Sullivan and M. C. Heffern, Org. Biomol. Chem., 2022, Advance Article , DOI: 10.1039/D2OB00436D

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