Transparent polycarbonate coated with CeO2 nanozymes repel Pseudomonas aeruginosa PA14 biofilms

Abstract

Highly transparent CeO₂/polycarbonate surfaces were fabricated that prevent adhesion, proliferation, and the spread of bacteria. CeO₂ nanoparticles with diameters of 10–15 nm and lengths of 100–200 nm for this application were prepared by oxidizing aqueous dispersions of Ce(OH)₃ with H₂O₂ in the presence of nitrilotriacetic acid (NTA) as the capping agent. The surface-functionalized water-dispersible CeO₂ nanorods showed high catalytic activity in the halogenation reactions, which makes them highly efficient functional mimics of haloperoxidases. These enzymes are used in nature to prevent the formation of biofilms through the halogenation of signaling compounds that interfere with bacterial cell–cell communication (“quorum sensing”). Bacteria-repellent CeO₂/polycarbonate plates were prepared by dip-coating plasma-treated polycarbonate plates in aqueous CeO₂ particle dispersions. The quasi-enzymatic activity of the CeO₂ coating was demonstrated using phenol red enzyme assays. The monolayer coating of CeO₂ nanorods (1.6 μg cm⁻²) and the bacteria repellent properties were demonstrated by atomic force microscopy, biofilm assays, and fluorescence measurements. The engineered polymer surfaces have the ability to repel biofilms as green antimicrobials on plastics, where H₂O₂ is present in humid environments such as automotive parts, greenhouses, or plastic containers for rainwater.