Issue 7, 2022

Rbm20 ablation is associated with changes in the expression of titin-interacting and metabolic proteins

Abstract

Dilated cardiomyopathy (DCM) is a major risk factor for developing heart failure and is often associated with an increased risk for life-threatening arrhythmia. Although numerous causal genes for DCM have been identified, RNA binding motif protein 20 (Rbm20) remains one of the few splicing factors that, when mutated or genetically ablated, leads to the development of DCM. In this study we sought to identify changes in the cardiac proteome in Rbm20 knockout (KO) rat hearts using global quantitative proteomics to gain insight into the molecular mechanisms precipitating the development of DCM in these rats. Our analysis identified changes in titin-interacting proteins involved in mechanical stretch-based signaling, as well as mitochondrial enzymes, which suggests that activation of pathological hypertrophy and altered mitochondrial metabolism and/or dysfunction, among other changes, contribute to the development of DCM in Rbm20 KO rats. Collectively, our findings provide the first report on changes in the cardiac proteome associated with genetic ablation of Rbm20.

Graphical abstract: Rbm20 ablation is associated with changes in the expression of titin-interacting and metabolic proteins

Supplementary files

Article information

Article type
Research Article
Submitted
15 Apr 2022
Accepted
21 Jun 2022
First published
22 Jun 2022

Mol. Omics, 2022,18, 627-634

Rbm20 ablation is associated with changes in the expression of titin-interacting and metabolic proteins

E. J. Larson, Z. R. Gregorich, Y. Zhang, B. H. Li, T. J. Aballo, J. A. Melby, Y. Ge and W. Guo, Mol. Omics, 2022, 18, 627 DOI: 10.1039/D2MO00115B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements