Issue 10, 2022

Actuated 3D microgels for single cell mechanobiology

Abstract

We present a new cell culture technology for large-scale mechanobiology studies capable of generating and applying optically controlled uniform compression on single cells in 3D. Mesenchymal stem cells (MSCs) are individually encapsulated inside an optically triggered nanoactuator-alginate hybrid biomaterial using microfluidics, and the encapsulating network isotropically compresses the cell upon activation by light. The favorable biomolecular properties of alginate allow cell culture in vitro up to a week. The mechanically active microgels are capable of generating up to 15% compressive strain and forces reaching 400 nN. As a proof of concept, we demonstrate the use of the mechanically active cell culture system in mechanobiology by subjecting singly encapsulated MSCs to optically generated isotropic compression and monitoring changes in intracellular calcium intensity.

Graphical abstract: Actuated 3D microgels for single cell mechanobiology

Associated articles

Supplementary files

Article information

Article type
Paper
Submitted
03 Mar 2022
Accepted
06 Apr 2022
First published
08 Apr 2022

Lab Chip, 2022,22, 1962-1970

Actuated 3D microgels for single cell mechanobiology

B. Özkale, J. Lou, E. Özelçi, A. Elosegui-Artola, C. M. Tringides, A. S. Mao, M. S. Sakar and D. J. Mooney, Lab Chip, 2022, 22, 1962 DOI: 10.1039/D2LC00203E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements