Issue 96, 2022
From the journal:

Chemical Communications

Quantum yield enhancement of firefly bioluminescence with biomolecular condensates†

Ryo Nishihara, ORCID logo ‡*ab   Yoshiki Kihara,‡a   Kazuki Niwa, ORCID logo a   Masahiro Mimura,ac   Shunsuke Tomita ORCID logo *a  and  Ryoji Kurita ORCID logo ac  

* Corresponding authors

a National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan

b Japan Science and Technology Agency (JST), PRESTO, 4-1-8, Honcho, Kawaguchi, Saitama 332-0012, Japan

c Faculty of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573, Japan

Abstract

The enzymatic luminescence reactions of fireflies are accelerated in the presence of biomolecular condensates comprising a positively charged peptide and ATP. We revealed that this acceleration is caused by the enrichment of reaction elements, local pH changes, and promotion of inhibitory intermediate dissociation, improving the bioluminescence quantum yield by approximately 10%.

Graphical abstract: Quantum yield enhancement of firefly bioluminescence with biomolecular condensates

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Article information

DOI
https://doi.org/10.1039/D2CC04919H
Article type
Communication
Submitted
06 Sep 2022
Accepted
28 Oct 2022
First published
28 Oct 2022

Chem. Commun., 2022,58, 13317-13320

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Quantum yield enhancement of firefly bioluminescence with biomolecular condensates

R. Nishihara, Y. Kihara, K. Niwa, M. Mimura, S. Tomita and R. Kurita, Chem. Commun., 2022, 58, 13317 DOI: 10.1039/D2CC04919H

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