Efficient O-demethylation of lignin monoaromatics using the peroxygenase activity of cytochrome P450 enzymes

Alix C. Harlington; Keith E. Shearwin; Stephen G. Bell; Fiona Whelan

doi:10.1039/D2CC04698A

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Efficient O-demethylation of lignin monoaromatics using the peroxygenase activity of cytochrome P450 enzymes†

Alix C. Harlington,

^a Keith E. Shearwin,

^a Stephen G. Bell

*^b and Fiona Whelan

*^a

Author affiliations

* Corresponding authors

^a Department of Molecular and Biomedical Science, University of Adelaide, Adelaide, SA 5005, Australia
E-mail: fiona.whelan@adelaide.edu.au

^b Department of Chemistry, University of Adelaide, Adelaide, SA 5005, Australia
E-mail: stephen.bell@adelaide.edu.au

Abstract

A crucial reaction in harnessing renewable carbon from lignin is O-demethylation. We demonstrate the selective O-demethylation of syringol and guaiacol using different cytochrome P450 enzymes. These can efficiently use hydrogen peroxide which, when compared to nicotinamide cofactor-dependent monooxygenases and synthetic methods, allows for cheap and clean O-demethylation of lignin-derived aromatics.

This article is part of the themed collection: Biocatalysis: A cross-journal collection

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Article information

DOI: https://doi.org/10.1039/D2CC04698A
Article type: Communication
Submitted: 25 Aug 2022
Accepted: 14 Oct 2022
First published: 08 Nov 2022
This article is Open Access

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Chem. Commun., 2022,58, 13321-13324

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Efficient O-demethylation of lignin monoaromatics using the peroxygenase activity of cytochrome P450 enzymes

A. C. Harlington, K. E. Shearwin, S. G. Bell and F. Whelan, Chem. Commun., 2022, 58, 13321 DOI: 10.1039/D2CC04698A

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Chemical Communications

Efficient O-demethylation of lignin monoaromatics using the peroxygenase activity of cytochrome P450 enzymes†

Abstract

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Efficient O-demethylation of lignin monoaromatics using the peroxygenase activity of cytochrome P450 enzymes

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