Carrier protein mediated cargo sensing in quorum signal synthases

Patrick D. Fischer; Abu Sayeed Chowdhury; Thomas Bartholow; Shibani Basu; Eric Baggs; Huel S. Cox; Srđan Matošin; Michael D. Burkart; Lisa Warner; Rajesh Nagarajan; Haribabu Arthanari

doi:10.1039/D2CC03551K

Carrier protein mediated cargo sensing in quorum signal synthases†

Patrick D. Fischer,^acd Abu Sayeed Chowdhury,

^f Thomas Bartholow,^e Shibani Basu,

^b Eric Baggs,^b Huel S. Cox, III,^ac Srđan Matošin,^ac Michael D. Burkart,

^e Lisa Warner,^b Rajesh Nagarajan

*^b and Haribabu Arthanari

*^ac

Author affiliations

* Corresponding authors

^a Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA
E-mail: hari@hms.harvard.edu

^b Department of Chemistry and Biochemistry, Boise State University, Boise, ID, USA
E-mail: rajnagarajan@boisestate.edu

^c Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA

^d Department of Pharmacy, Pharmaceutical and Medicinal Chemistry, Saarland University, Saarbrucken, Germany

^e Department of Chemistry and Biochemistry, University of California, San Diego, CA, USA

^f Biomolecular Sciences Graduate Program, Boise State University, Boise, ID, USA

Abstract

Acyl-homoserine lactone synthases make specific AHL quorum sensing signals to aid virulence in Gram-negative bacteria. Here, we use solution NMR spectroscopy to demonstrate that the carrier protein–enzyme interface accurately reveals substrate recognition mechanisms in two quorum signal synthases.

Chemical Communications

Carrier protein mediated cargo sensing in quorum signal synthases†

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