Issue 55, 2022
From the journal:

Chemical Communications

CATCH: high specific transcriptome-focused fusion gene variants discrimination†

Rui Yuan,‡ab   Xiaopeng Bai,‡c   Xiaolin Hu,b   Hong Zhang,bd   Changjun Hou, ORCID logo *a   Quanxin Long ORCID logo *c  and  Yang Luo ORCID logo *bef  

* Corresponding authors

a Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing 400044, P. R. China

b Center of Smart Laboratory and Molecular Medicine, School of Medicine, Chongqing University, Chongqing 400044, P. R. China

c Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing 400016, P. R. China

d Department of Clinical Laboratory, The Second Hospital of Shandong University, Jinan, Shandong 250033, P. R. China

e Department of Clinical Laboratory, Fuling Hospital, Chongqing University, Chongqing, P. R. China

f Department of Clinical Laboratory, Jiangjin Hospital, Chongqing University, Chongqing, P. R. China

Abstract

We develop a CRISPR-Cas13a triggered catalytic hairpin assembly (CATCH) approach for accurate and impartial identification of variants by integrating the fusion gene-selected recognition of CRISPR-Cas13a with collateral cleavage-assisted catalytic hairpin assembly amplification. This approach achieved an accuracy of 100% in a pilot experiment involving 34 clinical samples.

Graphical abstract: CATCH: high specific transcriptome-focused fusion gene variants discrimination

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Article information

DOI
https://doi.org/10.1039/D2CC02660K
Article type
Communication
Submitted
10 May 2022
Accepted
07 Jun 2022
First published
07 Jun 2022

Chem. Commun., 2022,58, 7618-7621

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CATCH: high specific transcriptome-focused fusion gene variants discrimination

R. Yuan, X. Bai, X. Hu, H. Zhang, C. Hou, Q. Long and Y. Luo, Chem. Commun., 2022, 58, 7618 DOI: 10.1039/D2CC02660K

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