Issue 36, 2022
From the journal:

Chemical Communications

Structure-based redesign of Fe(ii)/2-oxoglutarate-dependent oxygenase AndA to catalyze spiro-ring formation†

Takahiro Mori,‡*abc   Yu Nakashima,‡a   Heping Chen,‡a   Shotaro Hoshino,a   Takaaki Mitsuhashia  and  Ikuro Abe ORCID logo *ab  

* Corresponding authors

a Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, 113-0033, Japan
E-mail: tmori@mol.f.u-tokyo.ac.jp, abei@mol.f.u-tokyo.ac.jp

b Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan

c PRESTO, Japan Science and Technology Agency (JST), Kawaguchi, Saitama 332-0012, Japan

Abstract

Structure- and mechanism-based redesign of the Fe(II)/2-oxoglutarate-dependent oxygenase AndA was performed. The function of AndA was expanded to catalyze a spiro-ring formation reaction from an isomerization reaction. The redesigned AndA variants produced two unnatural novel spiro-ring containing compounds through two and three consecutive oxidation reactions.

Graphical abstract: Structure-based redesign of Fe(ii)/2-oxoglutarate-dependent oxygenase AndA to catalyze spiro-ring formation

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Article information

DOI
https://doi.org/10.1039/D2CC00736C
Article type
Communication
Submitted
07 Feb 2022
Accepted
10 Apr 2022
First published
11 Apr 2022

Chem. Commun., 2022,58, 5510-5513

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Structure-based redesign of Fe(II)/2-oxoglutarate-dependent oxygenase AndA to catalyze spiro-ring formation

T. Mori, Y. Nakashima, H. Chen, S. Hoshino, T. Mitsuhashi and I. Abe, Chem. Commun., 2022, 58, 5510 DOI: 10.1039/D2CC00736C

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