A PET-based fluorescent probe for monitoring labile Fe(II) pools in macrophage activations and ferroptosis

Wanjin Xing; Hang Xu; Huijuan Ma; Syed Ali Abbas Abedi; Shanshan Wang; Xingchen Zhang; Xiaogang Liu; Huan Xu; Wei Wang; Kaiyan Lou

doi:10.1039/D1CC06611K

A PET-based fluorescent probe for monitoring labile Fe(ii) pools in macrophage activations and ferroptosis†

Wanjin Xing,^a Hang Xu,^a Huijuan Ma,^a Syed Ali Abbas Abedi,^b Shanshan Wang,^a Xingchen Zhang,^a Xiaogang Liu,

^b Huan Xu,*^a Wei Wang

*^c and Kaiyan Lou

*^a

Author affiliations

* Corresponding authors

^a State Key Laboratory of Bioengineering Reactor, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, Shanghai Key Laboratory of New Drug Design, and Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science & Technology, 130 Meilong Road, Shanghai 200237, China
E-mail: xuhuan@ecust.edu.cn, kylou@ecust.edu.cn

^b Fluorescence Research Group, Singapore University of Technology and Design, 8 Somapah Road, Singapore 487372, Singapore

^c Department of Pharmacology and Toxicology and BIO5 Institute, University of Arizona, Tucson, AZ 85721-0207, USA
E-mail: wwang@pharmacy.arizona.edu

Abstract

A fluorescent probe (COU-LIP-1) for monitoring labile Fe(II) pools (LIP) with high selectivity and sensitivity was developed utilizing coumarin 343 as the fluorophore and 3-nitrophenylazanyl ester as both the reactive group and the fluorescence quenching group. Fe(II)-induced reductive cleavage of the N–O bond results in a turn-on response via a photo-induced photon transfer (PET) mechanism. The probe was applied for monitoring labile iron(II) changes in M1 and M2a macrophage activations and also erastin-induced ferroptosis, providing a powerful tool for selectively sensing LIP under both physiological and stressed conditions.

Chemical Communications

A PET-based fluorescent probe for monitoring labile Fe(ii) pools in macrophage activations and ferroptosis†

Abstract

Supplementary files

Article information

Download Citation

Permissions

A PET-based fluorescent probe for monitoring labile Fe(II) pools in macrophage activations and ferroptosis

Social activity

Search articles by author

Spotlight

Advertisements