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The increasing trend for non-expert users to undertake analytical measurements using an expanding range of chromatographic approaches can lead to the use of unsuitable separation methods and the generation of poor-quality data. Technical Brief AMCTB No. 107 introduced liquid chromatography and guidance on selection of the appropriate separation modality. This publication aims to provide a quick and easy to use educational tool for optimizing the mode of liquid chromatographic separation. It will be of value to both expert and non-expert users across the natural, life and physical sciences.

Graphical abstract: A periodic table for liquid chromatography separation modes

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