Issue 12, 2022

A portable blood lactate sensor with a non-immobilized enzyme for early sepsis diagnosis

Abstract

Early determination of blood lactate levels may accelerate the detection of sepsis, one of the most time-sensitive illnesses. We developed and validated a portable blood lactate detection kit for clinical screening that can measure early bedside lactate levels in intensive care unit (ICU) patients suspected of having sepsis. A TiO2 sol–G nanocomposite was prepared and coated on a screen-printed carbon electrode (SPCE) integrated with non-immobilized lactate oxidase (LOx) to produce a novel lactate biosensor with high sensitivity and high storage stability for human blood lactate measurement. The detection kit was based on an electrochemical technique and showed a wide linear range of 1–20 mM (R2 = 0.9937) with a low detection limit of 0.2 mM for lactate detection. This allowed for differentiating patient groups who may have sepsis using a cut-off level of 4 mM. The device was successfully implemented for blood lactate determination in critical patients, showing an accuracy range from 75% to 112%. This device provided high-precision and rapid quantitative information validated using a blood gas analyzer. Our detection kit might help to reduce the morbidity and mortality rates in severe sepsis and septic shock patients in community hospitals.

Graphical abstract: A portable blood lactate sensor with a non-immobilized enzyme for early sepsis diagnosis

Supplementary files

Article information

Article type
Paper
Submitted
04 Feb 2022
Accepted
18 Apr 2022
First published
26 May 2022

Analyst, 2022,147, 2819-2827

A portable blood lactate sensor with a non-immobilized enzyme for early sepsis diagnosis

P. Kiatamornrak, S. Boobphahom, T. Lertussavavivat, P. Rattanawaleedirojn, O. Chailapakul, N. Rodthongkum and N. Srisawat, Analyst, 2022, 147, 2819 DOI: 10.1039/D2AN00218C

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