Carbon dots up-regulate heme oxygenase-1 expression towards acute lung injury therapy

Abstract

Pneumonia is a kind of inflammation, which can cause high morbidity and mortality, and the treatment of pneumonia has received widespread attention. Heme oxygenase-1 (HMOX1) is a cell protective enzyme and can generate an anti-inflammatory response. Here, we demonstrate that degradable carbon dots (from L-ascorbic acid, CDs-1) can up-regulate the expression of HMOX1 in animal cells and tissues, which has a therapeutic effect on LPS-induced acute lung injury in mice. It was confirmed from in vitro experiments that CDs-1 could significantly up-regulate the expression of mRNA and the protein of HMOX1, which can increase the expression of HMOX1 by 5 times in a short time, decreasing the reactive oxygen species level in a cellular inflammation model induced by LPS. Furthermore, a series of in vivo comparative experiments show that CDs-1 could effectively treat acute lung injury and improve the survival rate of mice to 80%. Our work provides a practical way for the treatment of acute inflammation and the promising application of CDs in anti-inflammation.