Highly efficient nanomedicine from cationic antimicrobial peptide-protected Ag nanoclusters

Abstract

Designing the homogeneous assembly of the bio–nano interface to fine-tune the interactions between the nanoprobes and biological systems is of prime importance to improve the antimicrobial efficiency of nanomedicines. In this work, highly luminescent silver nanoclusters with the homogeneous conjugation of an antimicrobial peptide (referred to as Dpep-Ag NCs) were achieved via the reduction–decomposition–reduction process as a single package. The as-designed Dpep-Ag NCs inherited the two distinctive features of bactericides from the Ag⁺ species and the antimicrobial peptide of Dpep, and exhibited enhanced bacterial killing efficiency compared with other control groups including BSA-capped Ag NCs and the original antimicrobial peptide bactenecin (Opep)-protected Ag nanoparticles (Opep-Ag NPs). The ultrasmall size feature of Dpep-Ag NCs combined with the positively charged bactericidal tail allow a better interface and interaction with the cell membrane owing to the selective targeting of lipopolysaccharides in the Gram-negative bacteria and electrostatic interaction, facilitating the membrane permeability. Dpep-Ag NCs restrained the E. coli growth visibly and outperformed commercial Ag NPs (30 nm) with reduced (ca. 100-fold) minimal inhibitory concentration. The analysis of infected wound sizes and tissues treated with Dpep-Ag NCs in a murine model reveal obvious differences in the healing effect compared with the other counterparts, demonstrating its antibacterial efficiency in practical application.