Issue 30, 2021

Dancing with oils – the interaction of lipases with different oil/water interfaces

Abstract

The use of enzymes as biocatalysts in industrial applications has received much attention during the last few years. Lipases are widely employed in the food and cosmetic industry, for the synthesis of novel biomaterials and as a greener solution for the treatment of waste cooking oils (WCO). The latter topic has been widely explored with the use of enzymes from several origins and types, for the treatment of different used and non-used cooking oils. The experimental conditions of such works are also quite broad, hampering the detailed understanding of the process. In this work we present a detailed characterization of the interaction of several commonly used lipases with different types of vegetal oils and food fats through coarse-grained molecular dynamics simulations. First, the molecular details of the oil/water (O/W) mixtures, namely at the O/W interface, are described. The O/W interface was found to be enriched in triglyceride molecules with higher polarity. Then, the interaction of lipases with oil mixtures is characterized from different perspectives, including the identification of the most important protein residues for this process. The lipases from Thermomyces lanuginosus (TLL), Rhizomucor miehei (RML) and Candida antarctica (CALB) were found to bind to the O/W interface in a manner that makes the protein binding site more available for the oil molecules. These enzymes were also found to efficiently bind to the O/W interface of all oil mixtures, which in addition to reactivity factors, may explain the efficient applicability of these enzymes to a large variety of edible oils and WCO.

Graphical abstract: Dancing with oils – the interaction of lipases with different oil/water interfaces

Supplementary files

Article information

Article type
Paper
Submitted
21 Apr 2021
Accepted
02 Jun 2021
First published
02 Jun 2021

Soft Matter, 2021,17, 7086-7098

Dancing with oils – the interaction of lipases with different oil/water interfaces

H. A. L. Filipe, M. C. F. Almeida, R. R. Teixeira, M. I. M. Esteves, C. A. Henriques and F. E. Antunes, Soft Matter, 2021, 17, 7086 DOI: 10.1039/D1SM00590A

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