Loading and relaxation dynamics of a red blood cell

Abstract

We use mesoscale numerical simulations to investigate the unsteady dynamics of a single red blood cell (RBC) subjected to an external mechanical load. We carry out a detailed comparison between the loading (L) dynamics, following the imposition of the mechanical load on the RBC at rest, and the relaxation (R) dynamics, allowing the RBC to relax to its original shape after the sudden arrest of the mechanical load. Such a comparison is carried out by analyzing the characteristic times of the two corresponding dynamics, i.e., t_L and t_R. When the intensity of the mechanical load is small enough, the two kinds of dynamics are symmetrical (t_L ≈ t_R) and independent of the typology of mechanical load (intrinsic dynamics); otherwise, in marked contrast, an asymmetry is found, wherein the loading dynamics is typically faster than the relaxation one. This asymmetry manifests itself with non-universal characteristics, e.g., dependency on the applied load and/or on the viscoelastic properties of the RBC membrane. To deepen such a non-universal behaviour, we consider the viscosity of the erythrocyte membrane as a variable parameter and focus on three different typologies of mechanical load (mechanical stretching, shear flow, elongational flow): this allows to clarify how non-universality builds up in terms of the deformation and rotational contributions induced by the mechanical load on the membrane. Finally, we also investigate the effect of the elastic shear modulus on the characteristic times t_L and t_R. Our results provide crucial and quantitative information on the unsteady dynamics of RBC and its membrane response to the imposition/cessation of external mechanical loads.