Issue 23, 2021

A minimal hybridization chain reaction (HCR) system using peptide nucleic acids

Abstract

The HCR represents a powerful tool for amplification in DNA-based circuitry and sensing applications, yet requires the use of long DNA sequences to grant hairpin metastability. Here we describe a minimal HCR system based on peptide nucleic acids (PNAs). A system comprising a 5-mer stem and 5-mer loop/toehold hairpins was found to be suitable to achieve rapid amplification. These hairpins were shown to yield >10-fold amplification in 2 h and be suitable for the detection of a cancer biomarker on live cells. The use of γ-peg-modified PNA was found to be beneficial.

Graphical abstract: A minimal hybridization chain reaction (HCR) system using peptide nucleic acids

Supplementary files

Article information

Article type
Edge Article
Submitted
03 Mar 2021
Accepted
06 May 2021
First published
06 May 2021
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2021,12, 8218-8223

A minimal hybridization chain reaction (HCR) system using peptide nucleic acids

K. T. Kim, S. Angerani and N. Winssinger, Chem. Sci., 2021, 12, 8218 DOI: 10.1039/D1SC01269J

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