Superior anti-infective potential of eugenol–casein nanoparticles combined with polyethylene glycol against Colletotrichum musae infections

Abstract

The aim of this study was to improve the stability of eugenol–casein nanoparticles (EL–CS-NPs) through polyethylene glycol (PEG) modification. The results show that modifying the EL–CS-NPs with PEG after loading with eugenol (EL) gives PEG–EL–CS-NPs, with increased stability. The NPs modified with higher-molecular-weight PEG showed better stability. A CS/PEG ratio of 200 : 1 (w/w) yielded the NPs with the best stability. A PEG_{20 K}–EL–CS-NP dispersion remained stable in cold storage for over one year, and also exhibited stronger inhibitory effects against Colletotrichum musae inoculated on bananas than an EL–CS-NP dispersion, since it showed more prolonged sustained release of EL than the EL–CS-NP dispersion. Lyophilized PEG_{20 K}–EL–CS-NP powder showed better effectiveness against mold on bread than lyophilized EL–CS-NPs powder. Using PEG to modify CS-NPs shows potential for improving the stability of CS-NPs loaded with hydrophobic substances for delivery in the fields of food and agriculture.