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Isoishwarane synthase from Streptomyces lincolnensis

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Abstract

A new sesquiterpene synthase from Streptomyces lincolnensis was identified as isoishwarane synthase. The absolute configuration of its product was determined by an enantioselective deuteration strategy. The cyclisation mechanism from farnesyl diphosphate to isoishwarane was studied in incubation experiments with various isotopically labelled substrates and by site-directed mutagenesis. GC/MS analysis of all 15 isotopomers of (13C)isoishwarane also gave insights into the EI-MS fragmentation mechanism.

Graphical abstract: Isoishwarane synthase from Streptomyces lincolnensis

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Article information


Submitted
16 Dec 2020
Accepted
18 Jan 2021
First published
19 Jan 2021

Org. Chem. Front., 2021, Advance Article
Article type
Research Article

Isoishwarane synthase from Streptomyces lincolnensis

H. Xu, J. Rinkel and J. S. Dickschat, Org. Chem. Front., 2021, Advance Article , DOI: 10.1039/D0QO01583K

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