Issue 39, 2021

A reference scale of cucurbit[7]uril binding affinities


The accurate determination of ultra-high binding affinities in supramolecular host–guest chemistry is a challenging endeavour because direct binding titrations are generally limited to affinities <106 M−1 due to sensitivity constraints of common titration methods. To determine higher affinities, competitive titrations are usually performed, in which one compound with a well established binding affinity serves as a reference. Herein, we propose a reference scale for such competitive titrations with the host cucurbit[7]uril (CB7) comprising binding affinities in the range from 103 to 1015 M−1. The suggested reference compounds are commercially available and will aid in the future determination of CB7 binding affinities for stimuli-responsive host–guest systems.

Graphical abstract: A reference scale of cucurbit[7]uril binding affinities

Supplementary files

Article information

Article type
05 Jul 2021
03 Aug 2021
First published
03 Aug 2021
This article is Open Access
Creative Commons BY license

Org. Biomol. Chem., 2021,19, 8521-8529

A reference scale of cucurbit[7]uril binding affinities

M. A. Alnajjar, W. M. Nau and A. Hennig, Org. Biomol. Chem., 2021, 19, 8521 DOI: 10.1039/D1OB01304A

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