A promiscuous glycosyltransferase generates poly-β-1,4-glucan derivatives that facilitate mass spectrometry-based detection of cellulolytic enzymes

Gregory S. Bulmer; Ashley P. Mattey; Fabio Parmeggiani; Ryan Williams; Helene Ledru; Andrea Marchesi; Lisa S. Seibt; Peter Both; Kun Huang; M. Carmen Galan; Sabine L. Flitsch; Anthony P. Green; Jolanda M. van Munster

doi:10.1039/D1OB00971K

A promiscuous glycosyltransferase generates poly-β-1,4-glucan derivatives that facilitate mass spectrometry-based detection of cellulolytic enzymes†

Gregory S. Bulmer,

^a Ashley P. Mattey,

^a Fabio Parmeggiani,^ab Ryan Williams,^c Helene Ledru,^c Andrea Marchesi,

^a Lisa S. Seibt,

^a Peter Both,^a Kun Huang,^a M. Carmen Galan,

^c Sabine L. Flitsch,

^a Anthony P. Green

^a and Jolanda M. van Munster

*^ad

Author affiliations

* Corresponding authors

^a Manchester Institute of Biotechnology (MIB) & School of Natural Sciences, The University of Manchester, Manchester, UK
E-mail: jolanda.vanmunster@manchester.ac.uk, jolanda.van-munster@sruc.ac.uk

^b Department of Chemistry, Materials and Chemical Engineering “G. Natta”, Politecnico di Milano, Milano, Italy

^c School of Chemistry, University of Bristol, Bristol, UK

^d Scotland's Rural College, Central Faculty, Edinburgh, UK

Abstract

Promiscuous activity of a glycosyltransferase was exploited to polymerise glucose from UDP-glucose via the generation of β-1,4-glycosidic linkages. The biocatalyst was incorporated into biocatalytic cascades and chemo-enzymatic strategies to synthesise cello-oligosaccharides with tailored functionalities on a scale suitable for employment in mass spectrometry-based assays. The resulting glycan structures enabled reporting of the activity and selectivity of celluloltic enzymes.

This article is part of the themed collections: Chemical Biology in OBC and Catalysis & biocatalysis in OBC

Organic & Biomolecular Chemistry

A promiscuous glycosyltransferase generates poly-β-1,4-glucan derivatives that facilitate mass spectrometry-based detection of cellulolytic enzymes†

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A promiscuous glycosyltransferase generates poly-β-1,4-glucan derivatives that facilitate mass spectrometry-based detection of cellulolytic enzymes

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