Investigating eosin Y as a photocatalyst for the radical-dependent activity-based probing of deubiquitinating enzymes

Abstract

Eosin Y was assessed for its ability to induce a thiol–ene dependent protein–protein reaction in a metal-free, oxygen-tolerant, visible light mediated system. Protein–protein coupling efficiency under these mild conditions was comparable to previously reported UV-dependent conditions. The desired thiol–ene reaction was however limited within more complex biological systems.