Jump to main content
Jump to site search

Issue 6, 2021
Previous Article Next Article

A simple, high-throughput method of protein and label removal from extracellular vesicle samples

Author affiliations

Abstract

Evidence continues to increase of the clinical utility extracellular vesicles (EVs) as translational biomarkers. While a wide variety of EV isolation and purification methods have been implemented, few techniques are high-throughput and scalable for removing excess fluorescent reagents (e.g. dyes, antibodies). EVs are too small to be recovered from routine cell-processing procedures, such as filtration or centrifugation. The lack of suitable methods for removing unbound labels, especially in optical assays, is a major roadblock to accurate EV phenotyping and utilization of EV assays in a translational or clinical setting. Therefore, we developed a method for using a multi-modal resin, referred to as EV-Clean, to remove unbound labels from EV samples, and we demonstrate improvement in flow cytometric EV analysis with the use of this EV-Clean method.

Graphical abstract: A simple, high-throughput method of protein and label removal from extracellular vesicle samples

Back to tab navigation

Supplementary files

Article information


Submitted
03 Nov 2020
Accepted
08 Jan 2021
First published
05 Feb 2021

Nanoscale, 2021,13, 3737-3745
Article type
Paper

A simple, high-throughput method of protein and label removal from extracellular vesicle samples

J. A. Welsh, B. Killingsworth, J. Kepley, T. Traynor, K. McKinnon, J. Savage, D. Appel, K. Aldape, K. Camphausen, J. A. Berzofsky, A. R. Ivanov, I. H. Ghiran and J. C. Jones, Nanoscale, 2021, 13, 3737
DOI: 10.1039/D0NR07830A

Social activity

Search articles by author

Spotlight

Advertisements