Jump to main content
Jump to site search

Issue 1, 2021

Self-assembled chromogen-loaded polymeric cocoon for respiratory virus detection

Author affiliations

Abstract

Inspired by the self-assembly approach, in this work, the chromogen, 3,3′,5,5′-tetramethylbenzidine (TMB), was successfully co-precipitated in aqueous solution to form collective nanoparticles (NPs) of signal molecules (TMB-NPs). Utilizing poly(lactide-co-glycolide) (PLGA) in the molecular delivery approach, the formed emulsion nanovesicle (TMB-NPs@PLGA) exhibits an enrichment of the collective signal molecules in a single antibody–antigen conjugation. A specific antibody-conjugated TMB-NPs@PLGA forms an immunocomplex sandwich structure upon the addition of influenza virus (IV)/A. The addition of dimethyl sulfoxide (DMSO) dissolves the PLGA nanovesicles, releasing the encapsulated TMB-NPs. Sequentially, the TMB-NPs release TMB molecules upon the addition of DMSO. The released TMB is catalytically oxidized by H2O2 with self-assembled protein-inorganic nanoflowers, where copper nanoflowers (CuNFs) acted as the nanozyme. The developed immunoassay demonstrates high sensitivity for IV/A with a limit of detection (LOD) as low as 32.37 fg mL−1 and 54.97 fg mL−1 in buffer and serum, respectively. For practical needs, a clinically isolated IV/A/H3N2 and spike protein of SARS-CoV-2 were detected with the LODs of 17 pfu mL−1 and 143 fg mL−1, respectively. These results show the applicability of the advanced TMB-NPs@PLGA-based colorimetric sensor for the highly sensitive detection of airborne respiratory viruses.

Graphical abstract: Self-assembled chromogen-loaded polymeric cocoon for respiratory virus detection

Supplementary files

Article information


Submitted
26 Sep 2020
Accepted
30 Nov 2020
First published
08 Dec 2020

Nanoscale, 2021,13, 388-396
Article type
Paper

Self-assembled chromogen-loaded polymeric cocoon for respiratory virus detection

I. M. Khoris, A. B. Ganganboina, T. Suzuki and E. Y. Park, Nanoscale, 2021, 13, 388 DOI: 10.1039/D0NR06893D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements