Evaluation of substituent bioactivity and anion impact of linear and T-shaped silver(i) pyridinyl complexes as potential antiproliferative, antioxidant, antimicrobial agents and DNA- and BSA-binders†
Abstract
Transition metal-based drugs have shown to be good therapeutic agents and in the same effort, our work has continued the search for even better ones. Four pyridinyl Schiff bases (Ea–Ed) and their silver(I) nitrate, perchlorate, and triflate complexes (1–12) were synthesized and characterized by elemental analysis, FT-IR, ESI-MS, UV-Vis, 1H and 13C NMR spectroscopy, and single-crystal X-ray diffraction. The analysis confirmed a linear geometry around the Ag(I) center for all complexes in the solution, and a T-shaped geometry for complexes 10 and 12 in the solid-state. The newly synthesized compounds were screened for their in vitro anticancer, antioxidant, and antimicrobial activities. In addition, their interactions with calf thymus-DNA (CT-DNA) and bovine serum albumin (BSA) were also studied. Complexes possessing either methyl substituent or the thiazole moiety showed better in vitro antimicrobial activity against the tested bacteria, especially against Gram-negative bacteria. The anions ClO4− and CF3SO3− were observed to have some influence on the antimicrobial activities also. Effective scavenging activity in FRAP assay was observed for complexes with OH− or F− as substituents with IC50 values between 0.79 and 2.02 mg mL−1. The complexes were subjected to DNA and BSA binding studies. The complexes bound to CT-DNA either via intercalation or groove binding mode with significant binding constants for complexes whose ligands bore OH− substituent. The complexes displayed moderate to high binding affinity to BSA and again the influence of the fluoro and hydroxyl substituent or thiazole moiety were noted to influence the interactions. Complexes 4 and 8 were more cytotoxic against HELA (cervical cancer cell line) compared to MDA-MB231 (breast cancer cell line) and SHSY5Y (neuronal cancer cell line), with two-fold more potent cytotoxic activity than the reference cisplatin. The complexes, as such, might be potential anticervical cancer agents.