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Issue 14, 2021

Autonomous lab-on-paper for multiplexed, CRISPR-based diagnostics of SARS-CoV-2

Author affiliations

Abstract

The COVID-19 pandemic, caused by severe acute respiratory coronavirus 2 (SARS-CoV-2), has become a public health emergency and widely spread around the world. Rapid, accurate and early diagnosis of COVID-19 infection plays a crucial role in breaking this pandemic. However, the detection accuracy is limited for current single-gene diagnosis of SARS-CoV-2. Herein, we develop an autonomous lab-on-paper platform for multiplex gene diagnosis of SARS-CoV-2 by combining reverse transcription recombinase polymerase amplification (RT-RPA) and CRISPR–Cas12a detection. The autonomous lab-on-paper is capable of simultaneously detecting nucleoprotein (N) gene and spike (S) gene of SARS-CoV-2 virus as well as human housekeeping RNAse P gene (an internal control) in a single clinical sample. With the developed platform, 102 copies viral RNA per test can be detected within one hour. Also, the lab-on-paper platform has been used to detect 21 swab clinical samples and obtains a comparable performance to the conventional RT-PCR method. Thus, the developed lab-on-paper platform holds great potential for rapid, sensitive, reliable, multiple molecular diagnostics of COVID-19 and other infectious diseases in resource-limited settings.

Graphical abstract: Autonomous lab-on-paper for multiplexed, CRISPR-based diagnostics of SARS-CoV-2

Supplementary files

Article information


Submitted
06 Apr 2021
Accepted
02 Jun 2021
First published
02 Jun 2021

Lab Chip, 2021,21, 2730-2737
Article type
Paper

Autonomous lab-on-paper for multiplexed, CRISPR-based diagnostics of SARS-CoV-2

K. Yin, X. Ding, Z. Li, M. M. Sfeir, E. Ballesteros and C. Liu, Lab Chip, 2021, 21, 2730 DOI: 10.1039/D1LC00293G

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