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Issue 5, 2021
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Self-aligned sequential lateral field non-uniformities over channel depth for high throughput dielectrophoretic cell deflection

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Abstract

Dielectrophoresis (DEP) enables the separation of cells based on subtle subcellular phenotypic differences by controlling the frequency of the applied field. However, current electrode-based geometries extend over a limited depth of the sample channel, thereby reducing the throughput of the manipulated sample (sub-μL min−1 flow rates and <105 cells per mL). We present a flow through device with self-aligned sequential field non-uniformities extending laterally across the sample channel width (100 μm) that are created by metal patterned over the entire depth (50 μm) of the sample channel sidewall using a single lithography step. This enables single-cell streamlines to undergo progressive DEP deflection with minimal dependence on the cell starting position, its orientation versus the field and intercellular interactions. Phenotype-specific cell separation is validated (>μL min−1 flow and >106 cells per mL) using heterogeneous samples of healthy and glutaraldehyde-fixed red blood cells, with single-cell impedance cytometry showing that the DEP collected fractions are intact and exhibit electrical opacity differences consistent with their capacitance-based DEP crossover frequency. This geometry can address the vision of an “all electric” selective cell isolation and cytometry system for quantifying phenotypic heterogeneity of cellular systems.

Graphical abstract: Self-aligned sequential lateral field non-uniformities over channel depth for high throughput dielectrophoretic cell deflection

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Supplementary files

Article information


Submitted
01 Dec 2020
Accepted
21 Jan 2021
First published
25 Jan 2021

Lab Chip, 2021,21, 835-843
Article type
Paper

Self-aligned sequential lateral field non-uniformities over channel depth for high throughput dielectrophoretic cell deflection

X. Huang, K. Torres-Castro, W. Varhue, A. Salahi, A. Rasin, C. Honrado, A. Brown, J. Guler and N. S. Swami, Lab Chip, 2021, 21, 835 DOI: 10.1039/D0LC01211D

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