Issue 8, 2021

Microfluidic platform for serial mixing experiments with in operando nuclear magnetic resonance spectroscopy

Abstract

We present a microfluidic platform that allows in operando nuclear magnetic resonance (NMR) observation of serial mixing experiments. Gradually adding one reagent to another is a fundamental experimental modality, widely used to quantify equilibrium constants, for titrations, and in chemical kinetics studies. NMR provides a non-invasive means to quantify concentrations and to follow structural changes at the molecular level as a function of exchanged volume. Using active pneumatic valving on the microfluidic device directly inside an NMR spectrometer equipped with a transmission-line NMR microprobe, the system allows injection of aliquots and in situ mixing in a sample volume of less than 10 μL.

Graphical abstract: Microfluidic platform for serial mixing experiments with in operando nuclear magnetic resonance spectroscopy

Supplementary files

Article information

Article type
Paper
Submitted
30 Oct 2020
Accepted
02 Feb 2021
First published
04 Mar 2021
This article is Open Access
Creative Commons BY-NC license

Lab Chip, 2021,21, 1598-1603

Microfluidic platform for serial mixing experiments with in operando nuclear magnetic resonance spectroscopy

M. Plata, W. Hale, M. Sharma, J. M. Werner and M. Utz, Lab Chip, 2021, 21, 1598 DOI: 10.1039/D0LC01100B

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