Issue 1, 2021

Hyperspectral imaging-based exosome microarray for rapid molecular profiling of extracellular vesicles

Abstract

One of the challenges of exploiting extracellular vesicles (EVs) as a disease biomarker is to differentiate EVs released by similar cell types or phenotypes. This paper reports a high-throughput and label-free EV microarray technology to differentiate EVs by simultaneous characterization of a panel of EV membrane proteins. The EsupplV microarray platform, which consists of an array of antibodies printed on a photonic crystal biosensor and a microscopic hyperspectral imaging technique, can rapidly assess the binding of the EV membrane proteins with their corresponding antibodies. The EV microarray assay requires only a 2 μL sample volume and a detection time of less than 2 h. The EV microarray assay was validated by not only quantifying seven membrane proteins carried by macrophage-derived EVs but also distinguishing the EVs secreted by three macrophage phenotypes. In particular, the EV microarray technology can generate a molecular fingerprint of target EVs that can be used to identify the EVs' parental cells, and thus has utility for basic science research as well as for point-of-care disease diagnostics and therapeutics.

Graphical abstract: Hyperspectral imaging-based exosome microarray for rapid molecular profiling of extracellular vesicles

Supplementary files

Article information

Article type
Paper
Submitted
05 Oct 2020
Accepted
20 Nov 2020
First published
23 Nov 2020
This article is Open Access
Creative Commons BY-NC license

Lab Chip, 2021,21, 196-204

Hyperspectral imaging-based exosome microarray for rapid molecular profiling of extracellular vesicles

Y. Wang, Q. Zhang, W. Yuan, Y. Wang, H. J. Loghry, Z. Zhao, M. J. Kimber, L. Dong and M. Lu, Lab Chip, 2021, 21, 196 DOI: 10.1039/D0LC01006E

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