Jump to main content
Jump to site search

Issue 1, 2021
Previous Article Next Article

An integrated microfluidic system for early detection of sepsis-inducing bacteria

Author affiliations

Abstract

Since early diagnosis of sepsis may assist clinicians in initiating timely, effective, and prognosis-improving antibiotic therapy, we developed an integrated microfluidic chip (IMC) for rapid isolation of both Gram-positive and Gram-negative bacteria from blood. The device comprised a membrane-based filtration module (90 min operating time), a bacteria-capturing module using a micro-mixer containing magnetic beads coated with “flexible neck” regions of mannose-binding lectin proteins for bacteria capture (20 min), and a miniature polymerase chain reaction (PCR) module for bacteria identification (90 min via TaqMan® probe technology). The filter separated all white blood cells and 99.5% of red blood cells from bacteria, which were captured at rates approaching 85%. The PCR assay's limit of detection was 5 colony-forming units (CFU) per reaction, and the entire process was completed in only 4 h. Since this is far less than that for culture-based approaches, this IMC may serve as a promising device for detection of sepsis.

Graphical abstract: An integrated microfluidic system for early detection of sepsis-inducing bacteria

Back to tab navigation

Article information


Submitted
24 Sep 2020
Accepted
09 Nov 2020
First published
16 Nov 2020

Lab Chip, 2021,21, 113-121
Article type
Paper

An integrated microfluidic system for early detection of sepsis-inducing bacteria

Y. Fang, C. Wang, Y. Chen, C. Chien, F. Kuo, H. You, M. S. Lee and G. Lee, Lab Chip, 2021, 21, 113
DOI: 10.1039/D0LC00966K

Social activity

Search articles by author

Spotlight

Advertisements