Issue 45, 2021
From the journal:

Dalton Transactions

Direct detection of iron clusters in L ferritins through ESI-MS experiments†

Lara Massai,‡a   Silvia Ciambellotti, ORCID logo ‡abc   Lucrezia Cosottini,abc   Luigi Messori, ORCID logo *a   Paola Turano ORCID logo *abc  and  Alessandro Pratesi ORCID logo d  

* Corresponding authors

a Department of Chemistry “Ugo Schiff”, University of Florence, Via della Lastruccia 3-13, 50019 Sesto Fiorentino, FI, Italy
E-mail: luigi.messori@unifi.it

b Magnetic Resonance Center (CERM), University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, FI, Italy
E-mail: turano@cerm.unifi.it

c Consorzio Interuniversitario Risonanze Magnetiche di Metallo Proteine (C.I.R.M.M.P.), Via Luigi Sacconi 6, 50019 Sesto Fiorentino, FI, Italy

d Department of Chemistry and Industrial Chemistry (DCCI), University of Pisa, Via Giuseppe Moruzzi 13, 56124 Pisa, Italy

Abstract

Human cytoplasmic ferritins are heteropolymers of H and L subunits containing a catalytic ferroxidase center and a nucleation site for iron biomineralization, respectively. Here, ESI-MS successfully detected labile metal–protein interactions revealing the formation of tetra- and octa-iron clusters bound to L subunits, as previously underscored by X-ray crystallography.

Graphical abstract: Direct detection of iron clusters in L ferritins through ESI-MS experiments

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Article information

DOI
https://doi.org/10.1039/D1DT03106F
Article type
Communication
Submitted
13 Sep 2021
Accepted
19 Oct 2021
First published
19 Oct 2021

Dalton Trans., 2021,50, 16464-16467

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Direct detection of iron clusters in L ferritins through ESI-MS experiments

L. Massai, S. Ciambellotti, L. Cosottini, L. Messori, P. Turano and A. Pratesi, Dalton Trans., 2021, 50, 16464 DOI: 10.1039/D1DT03106F

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